Cytokine Reference Laboratory

The Cytokine Reference Laboratory is a CLIA-88 licensed facility specializing in the analysis of cytokines, chemokines, growth factors and other soluble mediators from a variety of biological sources from humans and many other animal species.

The Cytokine Reference Laboratory (CRL) has over 30 years of experience participating as a testing laboratory for a variety of clinical trials and research projects. It is highly experienced with immunoassays using ELISA (both conventional and multiplex), microfluidic (Ella platform) and fluorescence bead-based antibody systems (Luminex platform) that allow multiple assays to be performed on a very small amount of material. The CRL has flexibility for customization of testing from as few as 1 and up to 1000s of samples per project. A new single cell system is also offered for real-time functional secretome profiling (IsoLight platform).

The director of the laboratory is Dr. Angela Panoskaltsis-Mortari, Professor in the Departments of Pediatrics and Medicine. She is board-certified by the American Board of Medical Laboratory Immunology.

Cytokine Reference Laboratory Equipment

Expand all

Agilent BioTek Synergy LX

Synergy/LX (BioTek) multi-mode reader for fluorescence and chemiluminescence immunoassays

The Agilent BioTek Synergy LX multimode reader automates microplate assays including UV-VIS absorbance, fluorescence, and luminescence detection.

 

Agilent BioTek Synergy LX

Assist Plus Pipetting Robot

ASSIST PLUS Pipetting Robot from INTEGRA Biosciences

Pipetting Robot

 

The assist plus pipetting robot uses electronic pipettes to increase throughput, automate repetitive tasks and provide reproducibility.

More

 

Automated Cellular Proteomics Hub

The IsoLight is a hub for functional profiling of each cell type across a large assay menu of single-cell chip and software products. Run an entire highly-multiplexed proteomics workflow.  Please see FAQ for more information. 

IsoLight

BioTek ELx50

Two BioTek Elx50 automated plate washers

The ELx50 automated plate washer can be used for washing 96-well magnetic or polystyrene bead assays, such as those developed on the Luminex platform.  The washer’s modular platform allows for processing standard solid bottom plates used in many traditional ELISAs and cell based assays.

ELx50

Ella

Two Ella (Protein Simple) microfluidic, automated multiplex immunoassay machines on dedicated computers

Ella is a small machine for automated sandwich ELISA assays that utilizes microfluidics and analyte-specific antibody coated Glass Nano Reactors (GNRs) in specialized cartridges.  The Ella uses pneumatics and micro-valves to pump sample over three GNRs in series for each analyte.  Customized panels are available. More Information

ella

Epoch Microplate Spectrophotometer with Gen5 3.10 software

Agilent BioTek Epoch Microplate Spectrophotometer

The Agilent BioTek Epoch microplate spectrophotometer has monochromator-based optics, for a filter-free, wide wavelength range for UV-Vis absorbance measurements.

Agilent BioTek Epoch microplate spectrophotometer

Luminex 200 Instrument System

Luminex 200 system with sheath fluid delivery system with BioPlex Manager 6.0 software on a dedicated computer

 

The Luminex 200 Instrument System is a multiplexing platform capable of performing qualitative and quantitative analysis up to 48 different analytes in a single reaction volume on a flow cytometry-based platform.

Luminex 200 System

Magpix System

MAGPIX powered by Luminex XMAP Technology with xPonent software on a dedicated computer.

The Magpix is a versatile multiplexing platform capable of performing qualitative and quantitative analysis of proteins and nucleic acids in a variety of sample matrices.
 

Magpix

MultiFlo FX

MultiFlo FX is an automated multi-mode reagent dispenser for 6- to 1536-well microplates. The system incorporates several technologies in its modular design, such as Parallel Dispense, random access dispense (RAD) and Automated Media Exchange (AMX) modules.

MultiFloFX

Nuaire Class II, Type A2 Biological Safety Cabinet

NuAire Class II, Type A2 Biosafety Cabinets (BSC) offer personnel, product, and environmental protection to obtain product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product operations.

xMAP® INTELLIFLEX

The xMAP® INTELLIFLEX is a versatile flow-based multiplexing platform. 

  • Multiplexing: Up to 500 analytes in a single sample volume
  • Broad Dynamic Range >5.5 logs reducing repeats due to out-of-range values
  • Read Time: 96-well plate in <20 min; 384-well plate in <75 min

See FAQ

xmap-intelliflex

General Equipment

  • Certified Sanyo -80 freezer (monitored 24/7)
  • Certified +4 refrigerator (monitored 24/7)
  • +4 / -20 refrigerator / freezer
  • Universal 32R centrifuge
  • Fisher Scientific Incubater
  • Orbital plate shaker

Contact Us


612-626-7057

[email protected]

Visit Us


516 Delaware Street SE
13-127 Phillips-Wangensteen Building
Minneapolis, MN 55455

Mailing Address


Pediatrics BMT
MMC 391 Mayo
8366A (Campus Delivery Code)
420 Delaware St. SE
Minneapolis, MN 55455